Microscope Microbiology Work

Information on Microscope Microbiology Work. How to work with bacteria and E.Coli on a light microscope.

Microscope Microbiology Work

The preparation of culture plates and the methods of cultivation will be found in text-books on bacteriology. They are large square plates covered on one surface with nutrient gelatine, upon which isolated colonies of bacteria are growing. They should be examined with a low-power 1"-inch (32-mm.) Object glass, the mechanical stage having been removed from the surface of the stage for the purpose. The required colonies having been recognised, a morsel of the gelatine can be removed with a platinum needle, while the colony is in the field of the microscope, and can be smeared on a cover glass. A drop of distilled water having been added, it can be spread out on the cover glass and examined in a living state with the high-power dark-ground illuminator or dried in a spirit flame, stained and mounted on a 3 X 1-inch slip with a drop of Canada balsam.

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