Proper Microscope Illumination Setup

Proper Microscope Illumination Setup.

Proper Microscope Illumination Setup

It should now be observed whether the field is equally illuminated. Too much stress cannot be laid on this point, as it is one which is easily overlooked and is often the cause of considerable mischief. If the light comes through a window a well defined image of the sash is reflected by the mirror, and with a lower power objective this can easily be seen ; unless the mirror is correctly adjusted the field will appear to be crossed by dark bands. In the case of lamp light the flame is reflected and has a similar effect. With high powers this fault is not so easily noticed, and for this reason require the more care ; proper resolution may in this manner be partially or totally destroyed. The remedy is either by shifting the mirror or by varying its distance from the object. More information on this subject is given under the head of Sub-stage Illumination.

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